Primer
pT_tal-rev

Part:BBa_K4047001

Designed by: An Nguyen, Avery Imes   Group: iGEM21_MiamiU_OH   (2021-09-03)


primerT_tal-reverse


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 14
    Illegal PstI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 14
    Illegal PstI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 14
    Illegal PstI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


Long Description

This is the reverse primer that pairs with the forward primer BBa_K4047000 to amplify the gene for transaldolase (BBa_K4047034). The overlap sequence allows proper Gibson assembly with the pGEM-tal (BBa_K4047036) backbone. The annealing portion (all caps) allows annealing to the wildtype Synechococcus elongatus PCC 7942 genome for proper amplification.

Please note that this primer contains the EcoR1 and BamHI restriction enzyme sites, limiting their part compatability, but was registered here for future use that may be limited to these commonly available enzymes.

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Categories
Parameters
None